| Plasmid identification no. . | Description . | Source . |
|---|---|---|
| pRC5316 | MBP-Sec4p/pRS315 | This study |
| pRC5313D | MBP-Sec4p S8,10,11,201,204A/pRS315 | This study |
| pRC1820 | Sec4p/pRS315 | Collins laboratory |
| pRC3423 | Sec4p Q79L/pRS315 | Heger et al., 2011 |
| pRC5475 | Sec4p S29V/pRS315 | This study |
| pRC5464A | Sec4p S10,11A/pRS315 | This study |
| pRC5463A | ppSUMO Cdc5p/pRS426 | This study |
| pRC651 | GFP-Sec4p/pRS315 | Collins laboratory |
| pRC3000 | GFP-Sec4p S8,11,201,204D/pRS315 | Heger et al., 2011 |
| pRC5473 | GFP-Sec4p S8,10,11,201,204D/pRS315 | This study |
| pRC5472 | GFP-Sec4p S8,10,11,201,204A/pRS315 | This study |
| pRC5474 | Sec15p-MBP/pRS426 | This study |
| pRC5460 | Cdc5p-3xmCherry/pRS316 | This study |
| pRC5468 | SEC4 S2,8,10,11,201,204A T6A/pRS315 | This study |
| Plasmid identification no. . | Description . | Source . |
|---|---|---|
| pRC5316 | MBP-Sec4p/pRS315 | This study |
| pRC5313D | MBP-Sec4p S8,10,11,201,204A/pRS315 | This study |
| pRC1820 | Sec4p/pRS315 | Collins laboratory |
| pRC3423 | Sec4p Q79L/pRS315 | Heger et al., 2011 |
| pRC5475 | Sec4p S29V/pRS315 | This study |
| pRC5464A | Sec4p S10,11A/pRS315 | This study |
| pRC5463A | ppSUMO Cdc5p/pRS426 | This study |
| pRC651 | GFP-Sec4p/pRS315 | Collins laboratory |
| pRC3000 | GFP-Sec4p S8,11,201,204D/pRS315 | Heger et al., 2011 |
| pRC5473 | GFP-Sec4p S8,10,11,201,204D/pRS315 | This study |
| pRC5472 | GFP-Sec4p S8,10,11,201,204A/pRS315 | This study |
| pRC5474 | Sec15p-MBP/pRS426 | This study |
| pRC5460 | Cdc5p-3xmCherry/pRS316 | This study |
| pRC5468 | SEC4 S2,8,10,11,201,204A T6A/pRS315 | This study |