Rockefeller University Press

Table 1.

Regulators of the RAG GTPases involved in mediating amino acid signaling to mTORC1

Yeast orthologues	Mammalian orthologues	Component	Function	mTORC1 activation	References
EGO complex (Ego1-Ego2-Ego3); VAM6	Ragulator	LAMTOR1 (p18), LAMTOR2 (p14), LAMTOR3 (MP1), LAMTOR4 (C7orf59), LAMTOR5 (HBXIP)	GEF for Rag-A/B; tethers the Rags to the lysosome	Up	Binda et al., 2009; Nada et al., 2009; Sancak et al., 2010; Bar-Peled et al., 2012; Powis et al., 2015
SEACIT (Sea1, Npr2, Npr3)	GATOR1	DEPDC5, Nprl2, Nprl3	GAP for Rag-A/B	Down	Dokudovskaya and Rout, 2011; Bar-Peled et al., 2013; Panchaud et al., 2013
SEACAT (Sea4, Seh1, Sea2, Sea3, Sec13)	GATOR2	Mios, Seh1L, WDR24, WDR59, Sec13	Inhibits GATOR1	Up	Dokudovskaya et al., 2011; Bar-Peled et al., 2013; Panchaud et al., 2013
Lst4/Lst7	FOLLICULIN	FNIP1/2-FLCN	GAP for Rag-C/D	Up	Petit et al., 2013; Tsun et al., 2013; Péli-Gulli et al., 2015
	SH3BP4	SH3BP4	Inhibits Rag-A/B through direct binding	Down	Kim et al., 2012
	RNF152	RNF152	Ubiquitinates (K63) RagA, promotes interaction between RagA and GATOR1	Down	Deng et al., 2015
	SKP2	SKP2	Ubiquitinates (K63) RagA, promotes interaction between RagA and GATOR1	Down	Jin et al., 2015

Yeast orthologues	Mammalian orthologues	Component	Function	mTORC1 activation	References
EGO complex (Ego1-Ego2-Ego3); VAM6	Ragulator	LAMTOR1 (p18), LAMTOR2 (p14), LAMTOR3 (MP1), LAMTOR4 (C7orf59), LAMTOR5 (HBXIP)	GEF for Rag-A/B; tethers the Rags to the lysosome	Up	Binda et al., 2009; Nada et al., 2009; Sancak et al., 2010; Bar-Peled et al., 2012; Powis et al., 2015
SEACIT (Sea1, Npr2, Npr3)	GATOR1	DEPDC5, Nprl2, Nprl3	GAP for Rag-A/B	Down	Dokudovskaya and Rout, 2011; Bar-Peled et al., 2013; Panchaud et al., 2013
SEACAT (Sea4, Seh1, Sea2, Sea3, Sec13)	GATOR2	Mios, Seh1L, WDR24, WDR59, Sec13	Inhibits GATOR1	Up	Dokudovskaya et al., 2011; Bar-Peled et al., 2013; Panchaud et al., 2013
Lst4/Lst7	FOLLICULIN	FNIP1/2-FLCN	GAP for Rag-C/D	Up	Petit et al., 2013; Tsun et al., 2013; Péli-Gulli et al., 2015
	SH3BP4	SH3BP4	Inhibits Rag-A/B through direct binding	Down	Kim et al., 2012
	RNF152	RNF152	Ubiquitinates (K63) RagA, promotes interaction between RagA and GATOR1	Down	Deng et al., 2015
	SKP2	SKP2	Ubiquitinates (K63) RagA, promotes interaction between RagA and GATOR1	Down	Jin et al., 2015