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Table 5.

Face radii distributions (fiber to NPC + face to NPC) of WT MEFs with TPR, NUP153, and ELYS KD

siRNA	Lamin	Observed (nm)		Expected (nm)		Observed − expected (nm)		P value vs. expected		Observed − scrambled	P value vs. scrambled	No. of NPCs
KD	Labeled	Median	SD	Median	SD	Median	SD	Median	SD	Median (nm)	Median	N
Scrambled	LA	163.3	53.2	171.9	67.4	−8.6	−14.2	0.00				39,096
TPR KD	LA	154.3	49.6	164.3	59.9	−10.0	−10.4	0.00		−9.1	0.00	40,767
NUP153 KD	LA	155.9	48.3	162.8	56.6	−6.9	−8.3	0.00		−7.5	0.00	36,066
ELYS KD	LA	169.7	50.9	168.9	72.3	0.8	−21.3	0.38^a	0.00	6.3	0.00	21,521
Scrambled	LC	157.0	50.8	163.3	77.4	−6.4	−26.6	0.00				37,760
TPR KD	LC	150.8	47.0	161.5	103.3	−10.7	−56.2	0.00		−6.2	0.00	35,489
NUP153 KD	LC	152.8	47.3	157.8	58.9	−4.9	−11.7	0.00		−4.1	0.00	39,988
ELYS KD	LC	167.5	52.0	169.0	77.1	−1.5	−25.1	0.00		10.5	0.00	27,053
Scrambled	LB1	174.7	54.7	181.8	92.2	−7.1	−37.5	0.00				37,383
TPR KD	LB1	154.4	48.0	163.2	89.4	−8.9	−41.4	0.00		−20.3	0.00	40,899
NUP153 KD	LB1	173.6	56.1	178.1	67.1	−4.4	−11.0	0.00		−1.1	0.06	31,145
ELYS KD	LB1	157.1	48.8	162.1	70.6	−5.0	−21.7	0.00		−17.6	0.00	24,981
Scrambled	LB2	175.5	52.5	175.0	129.5	0.4	−76.9	0.22^a	0.95^a			35,444
TPR KD	LB2	159.0	47.7	158.7	147.2	0.3	−99.4	0.16^a	0.40^a	−16.4	0.00	36,974
NUP153 KD	LB2	170.6	55.2	166.5	68.9	4.0	−13.7	0.00		−4.9	0.00	31,628
ELYS KD	LB2	160.7	48.7	162.7	70.3	−2.0	−21.6	0.00		−14.8	0.00	25,215

siRNA	Lamin	Observed (nm)		Expected (nm)		Observed − expected (nm)		P value vs. expected		Observed − scrambled	P value vs. scrambled	No. of NPCs
KD	Labeled	Median	SD	Median	SD	Median	SD	Median	SD	Median (nm)	Median	N
Scrambled	LA	163.3	53.2	171.9	67.4	−8.6	−14.2	0.00				39,096
TPR KD	LA	154.3	49.6	164.3	59.9	−10.0	−10.4	0.00		−9.1	0.00	40,767
NUP153 KD	LA	155.9	48.3	162.8	56.6	−6.9	−8.3	0.00		−7.5	0.00	36,066
ELYS KD	LA	169.7	50.9	168.9	72.3	0.8	−21.3	0.38^a	0.00	6.3	0.00	21,521
Scrambled	LC	157.0	50.8	163.3	77.4	−6.4	−26.6	0.00				37,760
TPR KD	LC	150.8	47.0	161.5	103.3	−10.7	−56.2	0.00		−6.2	0.00	35,489
NUP153 KD	LC	152.8	47.3	157.8	58.9	−4.9	−11.7	0.00		−4.1	0.00	39,988
ELYS KD	LC	167.5	52.0	169.0	77.1	−1.5	−25.1	0.00		10.5	0.00	27,053
Scrambled	LB1	174.7	54.7	181.8	92.2	−7.1	−37.5	0.00				37,383
TPR KD	LB1	154.4	48.0	163.2	89.4	−8.9	−41.4	0.00		−20.3	0.00	40,899
NUP153 KD	LB1	173.6	56.1	178.1	67.1	−4.4	−11.0	0.00		−1.1	0.06	31,145
ELYS KD	LB1	157.1	48.8	162.1	70.6	−5.0	−21.7	0.00		−17.6	0.00	24,981
Scrambled	LB2	175.5	52.5	175.0	129.5	0.4	−76.9	0.22^a	0.95^a			35,444
TPR KD	LB2	159.0	47.7	158.7	147.2	0.3	−99.4	0.16^a	0.40^a	−16.4	0.00	36,974
NUP153 KD	LB2	170.6	55.2	166.5	68.9	4.0	−13.7	0.00		−4.9	0.00	31,628
ELYS KD	LB2	160.7	48.7	162.7	70.3	−2.0	−21.6	0.00		−14.8	0.00	25,215

Median and SD of the observed and expected sum of lamin fiber and lamin face to NPC distances, the difference between them, P values (see Materials and methods), and number of NPCs. The distributions were also compared with scrambled siRNA controls. The data in each row were collected from 20 cells. The Mann Whitney U test and Ansari-Bradley test were used as described in Materials and methods.

^aP values less than the Bonferroni corrected α value.

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