Rockefeller University Press

Table 1.

Protein combinations used to measure kinetics and steady-state tip complex composition with two regulators

Actin fluorophore	SNAP TOCA-1 fluorophore	Third protein	Number of time-lapse experiments	Number of time-lapse fields of view	Number of snapshot experiments	Number of snapshot fields of view
Alexa 568	Alexa 647	eGFP-Diaph3	3	9	6	10
Alexa 568	Alexa 647	eGFP-Fascin	2	6	5	46
Alexa 568	Alexa 488	SNAP–Ena Alexa 647	3	9	6	43
Alexa 647	Alexa 488	mKate-GBD	3	12	2	26
Alexa 647	Alexa 488	KCK–VASP Alexa 568	2	8	3	16
Alexa 568	Alexa 488	SNAP–N-WASP Alexa 647	5	17	0	0

Actin fluorophore	SNAP TOCA-1 fluorophore	Third protein	Number of time-lapse experiments	Number of time-lapse fields of view	Number of snapshot experiments	Number of snapshot fields of view
Alexa 568	Alexa 647	eGFP-Diaph3	3	9	6	10
Alexa 568	Alexa 647	eGFP-Fascin	2	6	5	46
Alexa 568	Alexa 488	SNAP–Ena Alexa 647	3	9	6	43
Alexa 647	Alexa 488	mKate-GBD	3	12	2	26
Alexa 647	Alexa 488	KCK–VASP Alexa 568	2	8	3	16
Alexa 568	Alexa 488	SNAP–N-WASP Alexa 647	5	17	0	0

eGFP, enhanced GFP.