Rockefeller University Press

Table 2.

Protein and fluorophore combinations used to measure steady-state tip complex compositions with three regulators

Protein 1	Fluorophore 1	Protein 2	Fluorophore 2	Protein 3	Fluorophore 3	Number of experiments	Fields of view
Diaph3	eGFP	GBD	mKate	SNAP–N-WASP	Alexa 647	4	22
Diaph3	eGFP	KCK-VASP	Alexa 568	SNAP-Ena	Alexa 647	4	27
Diaph3	eGFP	KCK-VASP	Alexa 568	SNAP–TOCA-1	Alexa 647	3	16
Fascin	eGFP	GBD	mKate	SNAP-Ena	Alexa 647	1	9
Fascin	eGFP	GBD	mKate	SNAP–TOCA-1	Alexa 647	4	41
Fascin	eGFP	GBD	mKate	SNAP-VASP	Alexa 647	4	40
Fascin	eGFP	KCK-VASP	Alexa 568	SNAP-Ena	Alexa 647	4	36
Fascin	eGFP	KCK-VASP	Alexa 568	SNAP–N-WASP	Alexa 647	4	36
SNAP–N-WASP	Alexa 488	GBD	mKate	SNAP–TOCA-1	Alexa 647	3	33
SNAP–N-WASP	Alexa 488	KCK-VASP	Alexa 568	SNAP-ENA	Alexa 647	4	42
SNAP–TOCA-1	Alexa 488	GBD	mKate	SNAP-ENA	Alexa 647	4	45
SNAP–TOCA-1	Alexa 488	GBD	mKate	SNAP–N-WASP	Alexa 647	4	39

Protein 1	Fluorophore 1	Protein 2	Fluorophore 2	Protein 3	Fluorophore 3	Number of experiments	Fields of view
Diaph3	eGFP	GBD	mKate	SNAP–N-WASP	Alexa 647	4	22
Diaph3	eGFP	KCK-VASP	Alexa 568	SNAP-Ena	Alexa 647	4	27
Diaph3	eGFP	KCK-VASP	Alexa 568	SNAP–TOCA-1	Alexa 647	3	16
Fascin	eGFP	GBD	mKate	SNAP-Ena	Alexa 647	1	9
Fascin	eGFP	GBD	mKate	SNAP–TOCA-1	Alexa 647	4	41
Fascin	eGFP	GBD	mKate	SNAP-VASP	Alexa 647	4	40
Fascin	eGFP	KCK-VASP	Alexa 568	SNAP-Ena	Alexa 647	4	36
Fascin	eGFP	KCK-VASP	Alexa 568	SNAP–N-WASP	Alexa 647	4	36
SNAP–N-WASP	Alexa 488	GBD	mKate	SNAP–TOCA-1	Alexa 647	3	33
SNAP–N-WASP	Alexa 488	KCK-VASP	Alexa 568	SNAP-ENA	Alexa 647	4	42
SNAP–TOCA-1	Alexa 488	GBD	mKate	SNAP-ENA	Alexa 647	4	45
SNAP–TOCA-1	Alexa 488	GBD	mKate	SNAP–N-WASP	Alexa 647	4	39

eGFP, enhanced GFP.