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Table 1.
Activation parameters
ChannelCurrent density (pA/pF)a (pA/pg)bV1/2-I (mV)ΔV1/2 BTX (mV)kact-I (mV)V1/2-II (mV)kact-II (mV)n
Bird Pum NaV1.4 119.5 ± 11.8 −23.4 ± 1.0  5.9 ± 0.3   20 
+BTX  −57.0 ± 0.7 −33.6 ± 1.2 3.8 ± 0.6 −26.0 ± 1.7 5.9 ± 0.8 
Pum NaV1.4 + Pum NaVβ2 86.5 ± 12.8 −24.9 ± 1.3  6.2 ± 0.5   15 
+BTX  −59.9 ± 1.9 −35.0 ± 2.3 3.5 ± 0.5 −28.0 ± 1.4 5.7 ± 0.6 
Pum NaV1.5 17.1 ± 3.5 −22.4 ± 1.6  8.6 ± 0.3   
+BTX  −59.8 ± 0.8 −37.4 ± 1.8 5.5 ± 1.9 −25.0 ± 2.3 11.3 ± 0.7 
Pum NaV1.4 N432T (DI) 107.5 ± 26.9 −20.2 ± 1.9  6.7 ± 0.5   
+BTX  −51.0 ± 0.9 −30.8 ± 2.1 4.4 ± 0.4 −9.9 ± 6.1 7.4 ± 1.4 10 
Pum NaV1.4 N830T (DII) 8.1 ± 1.5 −22.4 ± 1.0  9.2 ± 0.5   16 
+BTX  −54.0 ± 1.7 −31.6 ± 2.0 7.6 ± 1.0 −22.9 ± 1.8 5.2 ± 0.9 
Pum NaV1.4 N1306T (DIII) 103.7 ± 29.4 −26.7 ± 1.0  6.4 ± 0.3   10 
+BTX  −63.6 ± 1.0 −36.9 ± 1.4 3.7 ± 0.6 −24.8 ± 1.2 8.5 ± 1.3 
Pum NaV1.4 N1609T (DIV) 24.6 ± 10.8 −2.9 ± 1.1  9.6 ± 1.0   
+BTX  −14.3 ± 3.0 −11.4 ± 3.2 9.7 ± 2.1 N/A N/A 
Pum NaV1.4 N1609A (DIV) 28.9 ± 4.6 −3.8 ± 1.3  10.7 ± 0.4   21 
+BTX  −45.1 ± 2.2 −41.3 ± 2.6 9.1 ± 0.8 −9.6 ± 2.0 8.6 ± 1.1 14 
Human Hs NaV1.4 70.9 ± 13.8 −13.3 ± 1.6  6.1 ± 0.4   13 
+BTX  −49.2 ± 0.9 −35.9 ± 1.8 5.0 ± 0.4 −19.0 ± 1.9 5.2 ± 0.5 
Hs NaV1.4 N1591T (DIV) 31.7 ± 13.4 3.6 ± 2.2  10.9 ± 1.1   
+BTX  −10.6 ± 5.0 −14.2 ± 5.5 9.7 ± 0.6 N/A N/A 
Rat Rn NaV1.4 445.0 ± 125.1 −23.1 ± 0.3  7.3 ± 0.3   
+BTX  −68.7 ± 0.2 −45.6 ± 0.4 4.0 ± 0.1   
Rn NaV1.4 N1584T (DiV) 43.2 ± 40.1 −5.1 ± 0.5  8.6 ± 0.3   
+BTX  −45.4 ± 2.7 −40.3 ± 2.8 9.6 ± 1.1 −17.0 ± 1.2 13.0 ± 0.9 
Poison frog Pt NaV1.4 26.2 ± 5.9 −24.3 ± 1.4  7.6 ± 0.3   
Oocytes 669.7 ± 120.4 −16.2 ± 2.0  4.3 ± 0.6   11 
+BTX  −54.3 ± 1.6 −30.0 ± 2.1 6.3 ± 0.8 −20.0 ± 2.2 9.1 ± 1.5 
+BTX oocytes  −49.3 ± 2.5 −33.1 ± 3.2 4.8 ± 0.7   
Pt NaV1.4 (N1600T) (DIV) 24.1 ± 3.9 −23.8 ± 1.2  9.0 ± 0.3   10 
+BTX  −54.6 ± 2.2 −30.8 ± 2.5 5.6 ± 0.8 −19.0 ± 6.7 11.7 ± 2.0 
Dt NaV1.4 11.1 ± 1.8 −17.1 ± 1.6  7.7 ± 0.4   13 
CHO cells 18.7 ± 4.4 −9.7 ± 1.5  8.0 ± 0.4   11 
oocytes 108.1 ± 24.3 −19.6 ± 2.5  5.1 ± 0.6   10 
+BTX  −55.0 ± 1.2 −37.9 ± 2.0 5.8 ± 1.4 −17.4 ± 2.7 8.2 ± 0.8 
+BTX CHO cells  −39.3 ± 1.9 −29.6 ± 2.4 6.7 ± 0.5 −0.2 ± 2.1 8.5 ± 0.9 
+BTX oocytes  −57.4 ± 5.6 −37.8 ± 6.1 5.2 ± 0.7   
Dt NaV1.4 (N1600T) (DIV) 10.0 ± 2.0 −17.2 ± 1.2  10.7 ± 0.9   
CHO cells 13.4 ± 1.4 −5.5 ± 1.2  8.0 ± 0.4   12 
+BTX  −55.1 ± 1.4 −37.8 ± 1.8 6.0 ± 0.5 −18.0 ± 3.1 9.8 ± 1.3 
+BTX CHO cells  −40.1 ± 2.4 −34.6 ± 2.7 5.9 ± 0.8 1.4 ± 3.1 7.0 ± 1.0 
ChannelCurrent density (pA/pF)a (pA/pg)bV1/2-I (mV)ΔV1/2 BTX (mV)kact-I (mV)V1/2-II (mV)kact-II (mV)n
Bird Pum NaV1.4 119.5 ± 11.8 −23.4 ± 1.0  5.9 ± 0.3   20 
+BTX  −57.0 ± 0.7 −33.6 ± 1.2 3.8 ± 0.6 −26.0 ± 1.7 5.9 ± 0.8 
Pum NaV1.4 + Pum NaVβ2 86.5 ± 12.8 −24.9 ± 1.3  6.2 ± 0.5   15 
+BTX  −59.9 ± 1.9 −35.0 ± 2.3 3.5 ± 0.5 −28.0 ± 1.4 5.7 ± 0.6 
Pum NaV1.5 17.1 ± 3.5 −22.4 ± 1.6  8.6 ± 0.3   
+BTX  −59.8 ± 0.8 −37.4 ± 1.8 5.5 ± 1.9 −25.0 ± 2.3 11.3 ± 0.7 
Pum NaV1.4 N432T (DI) 107.5 ± 26.9 −20.2 ± 1.9  6.7 ± 0.5   
+BTX  −51.0 ± 0.9 −30.8 ± 2.1 4.4 ± 0.4 −9.9 ± 6.1 7.4 ± 1.4 10 
Pum NaV1.4 N830T (DII) 8.1 ± 1.5 −22.4 ± 1.0  9.2 ± 0.5   16 
+BTX  −54.0 ± 1.7 −31.6 ± 2.0 7.6 ± 1.0 −22.9 ± 1.8 5.2 ± 0.9 
Pum NaV1.4 N1306T (DIII) 103.7 ± 29.4 −26.7 ± 1.0  6.4 ± 0.3   10 
+BTX  −63.6 ± 1.0 −36.9 ± 1.4 3.7 ± 0.6 −24.8 ± 1.2 8.5 ± 1.3 
Pum NaV1.4 N1609T (DIV) 24.6 ± 10.8 −2.9 ± 1.1  9.6 ± 1.0   
+BTX  −14.3 ± 3.0 −11.4 ± 3.2 9.7 ± 2.1 N/A N/A 
Pum NaV1.4 N1609A (DIV) 28.9 ± 4.6 −3.8 ± 1.3  10.7 ± 0.4   21 
+BTX  −45.1 ± 2.2 −41.3 ± 2.6 9.1 ± 0.8 −9.6 ± 2.0 8.6 ± 1.1 14 
Human Hs NaV1.4 70.9 ± 13.8 −13.3 ± 1.6  6.1 ± 0.4   13 
+BTX  −49.2 ± 0.9 −35.9 ± 1.8 5.0 ± 0.4 −19.0 ± 1.9 5.2 ± 0.5 
Hs NaV1.4 N1591T (DIV) 31.7 ± 13.4 3.6 ± 2.2  10.9 ± 1.1   
+BTX  −10.6 ± 5.0 −14.2 ± 5.5 9.7 ± 0.6 N/A N/A 
Rat Rn NaV1.4 445.0 ± 125.1 −23.1 ± 0.3  7.3 ± 0.3   
+BTX  −68.7 ± 0.2 −45.6 ± 0.4 4.0 ± 0.1   
Rn NaV1.4 N1584T (DiV) 43.2 ± 40.1 −5.1 ± 0.5  8.6 ± 0.3   
+BTX  −45.4 ± 2.7 −40.3 ± 2.8 9.6 ± 1.1 −17.0 ± 1.2 13.0 ± 0.9 
Poison frog Pt NaV1.4 26.2 ± 5.9 −24.3 ± 1.4  7.6 ± 0.3   
Oocytes 669.7 ± 120.4 −16.2 ± 2.0  4.3 ± 0.6   11 
+BTX  −54.3 ± 1.6 −30.0 ± 2.1 6.3 ± 0.8 −20.0 ± 2.2 9.1 ± 1.5 
+BTX oocytes  −49.3 ± 2.5 −33.1 ± 3.2 4.8 ± 0.7   
Pt NaV1.4 (N1600T) (DIV) 24.1 ± 3.9 −23.8 ± 1.2  9.0 ± 0.3   10 
+BTX  −54.6 ± 2.2 −30.8 ± 2.5 5.6 ± 0.8 −19.0 ± 6.7 11.7 ± 2.0 
Dt NaV1.4 11.1 ± 1.8 −17.1 ± 1.6  7.7 ± 0.4   13 
CHO cells 18.7 ± 4.4 −9.7 ± 1.5  8.0 ± 0.4   11 
oocytes 108.1 ± 24.3 −19.6 ± 2.5  5.1 ± 0.6   10 
+BTX  −55.0 ± 1.2 −37.9 ± 2.0 5.8 ± 1.4 −17.4 ± 2.7 8.2 ± 0.8 
+BTX CHO cells  −39.3 ± 1.9 −29.6 ± 2.4 6.7 ± 0.5 −0.2 ± 2.1 8.5 ± 0.9 
+BTX oocytes  −57.4 ± 5.6 −37.8 ± 6.1 5.2 ± 0.7   
Dt NaV1.4 (N1600T) (DIV) 10.0 ± 2.0 −17.2 ± 1.2  10.7 ± 0.9   
CHO cells 13.4 ± 1.4 −5.5 ± 1.2  8.0 ± 0.4   12 
+BTX  −55.1 ± 1.4 −37.8 ± 1.8 6.0 ± 0.5 −18.0 ± 3.1 9.8 ± 1.3 
+BTX CHO cells  −40.1 ± 2.4 −34.6 ± 2.7 5.9 ± 0.8 1.4 ± 3.1 7.0 ± 1.0 

Current densities for mock-transfected HEK293 and CHO cells were 1.3 ± 0.2 and 1.3 ± 0.3 pA/pF, n = 10 and 14, respectively. V1/2-I and V1/2-II are the half-activation potential. kactI an kactII are the slope factors. Values having “I” only indicate those fit by a single Boltzmann equation. Values having “I” and “II” indicate in cases where the data are fit by a double Boltzmann equation.

a

Values for mammalian cells.

b

Values for oocytes.

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