Skip to Main Content
Table 5.
Surface creation parameters for Video 1, Video 2, and Video 3 
Surface/ spots nameSource channelSmoothing: surface details (μm)Background subtraction: diameter of largest sphere (μm)Absolute intensity threshold (μm)Split touching objects: split seed diameter (μm)Quality thresholdVoxel number threshold
MHCII surfaces MHCII AF555 0.5 15 8.3 10 6.3 1,500 
S. Tm surfaces S. Tm. AF647 0.5 4.3 
Surface/ spots nameSource channelSmoothing: surface details (μm)Background subtraction: diameter of largest sphere (μm)Absolute intensity threshold (μm)Split touching objects: split seed diameter (μm)Quality thresholdVoxel number threshold
MHCII surfaces MHCII AF555 0.5 15 8.3 10 6.3 1,500 
S. Tm surfaces S. Tm. AF647 0.5 4.3 
Close Modal

or Create an Account

Close Modal
Close Modal