Figure S4.
Regulation of mitochondrial and lysosomal contacts with the ER. (A and B) Confocal microscopy image of ER contacts with mitochondria (inset in B) in live HeLa cells (ER, red [mCherry-ER]; mitochondria, green [mEmerald-TOMM20]). Scale bars, 5 μm (A); 1 μm (B). (C–E) Effect of TBC1D15 (WT vs. D397A), Fis1 (WT vs. LA), and Drp1 (WT vs. K38A) on ER-mitochondria contacts. C, n = 45 cells (TBC1D15(WT)); n = 57 cells (TBC1D15(D397A)); D, n = 33 cells (Fis1(WT)); n = 27 cells (Fis1(LA)); E, n = 29 cells (Drp1(WT)); n = 38 cells (Drp1(K38A)). (F and G) Confocal microscopy image of ER contacts with lysosomes (inset in G) in live HeLa cells (ER, red [mCherry-ER]; lysosome, green [mTagBFP2-Lysosomes-20]). Scale bars, 5 μm (F); 1 μm (G). (H–J) Effect of TBC1D15 (WT vs. D397A), Fis1 (WT vs. LA), and Drp1 (WT vs. K38A) on ER-lysosome contacts. H, n = 45 cells (TBC1D15(WT)); n = 57 cells (TBC1D15(D397A)); I, n = 33 cells (Fis1(WT)); n = 27 cells (Fis1(LA)); J, n = 19 cells (Drp1(WT)); n = 23 cells (Drp1(K38A)). Mean ± SEM; unpaired two-tailed t test (C–E and H–J); N.S., not significant (D, E, H, and I); *, P = 0.0157 (C); *, P = 0.0432 (J).