Figure S3.
Spontaneous Ca
2+
transients in WT and R2509C mutant primary myocytes. (A–C) Ca2+ transients in Cal-520–loaded primary myocytes at 3 d after differentiation from WT (A), R2509C-Het (B), and R2509C-Hom (C) mice were measured for 200 s with no stimulation. Different color lines indicate individual signals. Spontaneous Ca2+ transients were observed in primary myocytes from WT (Video 4) and R2509C-Het (Video 5) mice, but not in those from R2509C-Hom (Video 6) mice. (D) Number of myocytes demonstrating spontaneous Ca2+ transients out of total number of myocytes in the field of view during each observation, in which Ca2+ transients were measured for 200 s with no stimulation. The number at the top of bar indicates total number of positive myocytes out of total number of myocytes. Primary myocytes were cultured from three mice (N = 3) in each group (WT, R2509C-Het, and R2509C-Hom). Data are means ± SD (n = 4−5 observations) and analyzed by one-way ANOVA with Tukey’s test.