Figure S4.
Syx1 and Syx4 interact with Rop. (A) Confocal images of fat body expressing Rop-GFP alone (control) or co-expressing Rop-GFP with Syx1-FLAG-HA, Syx4-FLAG-HA, Syx7-FLAG-HA, and Syx17-FLAG-HA, all driven by BM-40-SPARC-GAL4. Rectangular insets are shown magnified below. (B) Quantification of the recruitment of Rop-GFP to the plasma membrane by Syx1, Syx4, Syx7, and Syx17. Each dot represents the ratio in one cell of Rop-GFP signal intensity between plasma membrane and cytoplasm, measured in images like those in A. n = 10. Horizontal lines mark the mean. P values from unpaired t tests are indicated for differences with the control. n.s., P > 0.05; ****, P < 0.0001. (C) Confocal images of fat body from wild-type, BM-40-SPARC>Ropi, >Vps20i, and >VhaPPA1-1i L3 larvae (feeding stage) expressing Rab11-GFP (green). (D) Confocal images of fat body from wild-type, BM-40-SPARC>Ropi, >Vps20i, and >VhaPPA1-1i L3 larvae (feeding stage) expressing trans-Golgi marker GalT-TagRFP (magenta). (E) Confocal images of fat body from wild-type, BM-40-SPARC>Rab11i, >Ropi, >Syx1i, >Syx4i, and double >Syx1i+Syx4i L3 larvae (feeding stage) stained with an antibody against trans-Golgi protein Golgin245 (cyan). (F) Trans-Golgi size measured in images like those in E. Data are presented as violin plots where quartiles are marked. Dots represent individual punctum size measurements (n = 100 per genotype).