Figure S5.
Changing Vap33 expression does not affect dendrites in Vps54
KO/KO
neurons Quantification of total dendrite branch length of class IV da neurons in 7-d-old adults. Analyzed by one-way ANOVA with Tukey’s post-test. Data presented as mean ± SD. (A) Effect of overexpression of Vap33 in c4da neurons. +/+ vs. Vps54KO/KO ***P = 0.0007. +/+ vs. ppk > Vap33 *P = 0.0265. +/+ vs. Vps54KO/KO; ppk > Vap33 ***P = 0.0001. Vps54KO/KO vs. Vps54KO/KO; ppk > Vap33 n.s. P = 0.9984. (B) Effect of Vap33 knockdown in c4da neurons. RNAi control vs. Vps54KO/KO; RNAi control ****P < 0.0001. RNAi control vs. Vps54KO/KO; Vap33 RNAi ***P = 0.0009. Vps54KO/KO; RNAi control vs. Vps54KO/KO; Vap33 RNAi n.s. P = 0.8367. N = 8–10 independent neurons for all genotypes except +/+ and Vps54KO/KO in A (n = 6 neurons for both).