Figure 3.
Ultrastructural analysis of the tibial nerve. (A) Example electronmicrographs from the tibial nerve of a WT Tmem150c+/+ (top) and Tmem150c−/− mouse (bottom). Scale bar = 5 μm. (B) Quantification of axon numbers revealed no loss of myelinated A-fibers (two-way ANOVA, P = 0.99) or unmyelinated C-fibers (two-way ANOVA, P = 0.95). (C) Measurements of the G-ratio a measure of myelin thickness showed no difference between genotypes (unpaired t test, P = 0.16). (D) Quantification of myelin thickness across all axon sizes (binned by myelin thickness) revealed no statistically significant differences between nerves taken from control Tmem150c+/+ (grey) and Tmem150c−/− mice (blue; two-way ANOVA, P = 0.92 for genotype), means ± SEM.