Figure S3.
Preventing MEN activation or nuclear movement into the bud stops spindle collapse in esp1-1 cells.
cdc20-AID (Ry7873), esp1-1 (Ry9490), esp1-1 dyn1∆ (Ry9516), esp1-1 cdc15-as1 (Ry9512), esp1-1 cdc5-as1 (Ry9134), esp1-1 cdc14-1 (Ry9131), esp1-1 cdc14-1 cdc5-as1 (Ry9128), and cdc14-1 cdc5-as1 (Ry1602) cells were arrested in G1 and released into restrictive conditions for the alleles used. Samples were taken at the indicated time points to determine the percentage of cells with short bipolar spindles (n = 100 cells).