Figure 10.
ADAMTS9/20 promotes type IV collagen assembly at the B-LINK. (A) A diagram showing when animals were photobleached and imaged. (B) Left: Fluorescence images of a1-type IV collagen::mRuby2 on control (L4440 empty vector, n = 10 each) and ADAMTS9/20 (gon-1, n = 10 each) RNAi. Right: Mean fluorescence intensity of each component quantified. ****P < 0.0001, unpaired two-tailed Student’s t test. (C) Left: Fluorescence images of ADAMTS9/20::mNG on control (T444T empty vector, n = 10 each) and hemicentin (him-4, n = 10 each) RNAi. Right: Mean fluorescence intensity of each component quantified. ****P < 0.0001, unpaired two-tailed Student’s t test. (D) Left: Fluorescence images of α1-type IV collagen::mRuby2 before photobleaching, immediately after photobleaching, and 2 h post-photobleaching in young adult animals. Images were corrected to account for general bleaching over the course of the experiment (Materials and methods). Dotted white box indicates bleached region. Right: A boxplot showing the mean percent recovery of α1-type IV collagen::mRuby2 in the young adult B-LINK after 2 h (n = 11 control, n = 8 ADAMTS9/20 RNAi). Values were normalized to an unbleached region of the B-LINK to account for general photobleaching. **P < 0.01, unpaired two-tailed Student’s t test. (E) A model for assembly and role of different matrix components at the utse–seam B-LINK. Box edges in boxplots depict the 25th and 75th percentiles, the line in the box indicates the median value, and whiskers mark the minimum and maximum values. Scale bars, 10 µm.