Figure 2.
Rapid accumulation of mEmerald-LC at the rupture sites. During time-lapse imaging of exogenous LA, LC, LB1, and LB2 and endogenous LA and LC with 10 s intervals, a 2-μm diameter spot was laser-microirradiated to induce NE rupture (yellow arrowheads). (A) Dynamics of mEmerald-LA, LC, LB1, and LB2 in response to NE rupture in MEFs. (B) Fluorescence intensities of these mEmerald-lamins at the rupture sites were measured and normalized to the initial intensities. The graph represents means ± SEM (n = 20 cells from two independent experiments; **, P < 0.001 from others by a linear mixed model). (C) Dynamics of sfGFP-DARPin-LA6 and NLS-sfCherry in response to NE rupture in MEFs. (D) The sfGFP-DARPin-LA6 intensity at the rupture sites and NLS-sfCherry intensity in the nucleoplasm were measured and the relative intensities are plotted (means ± SEM; n = 20 cells from two independent experiments). (E) Dynamics of sfGFP-DARPin-LA6 in response to NE rupture in MEFs expressing shRNAs, scrambled control (shScr), shLA or shLC. (F) The sfGFP-DARPin-LA6 intensity at the rupture sites was measured and the relative intensities are plotted (means ± SEM; n = 10 cells; **, P < 0.001; ns, P > 0.05 from control by a linear mixed model). (A, C, and E) Bars: 5 μm (the first column) and 2 μm (the second to fifth columns).