Figure 6.
A crucial role of v-ATPase and ribosomal subunits in mitochondrial surveillance and mitochondrial stress-induced longevity. (A) Representative brightfield pictures of wild-type (N2), isp-1(qm150), or clk-1(qm30) worms fed with control (ev), vha-1 (20%), vha-4 (20%), vha-16 (10%), or vha-19 (20%) RNAi since maternal L4 stage. The developmental stage and body length of the F1 progeny were quantified on Day 4 after hatching (n = 25 worms for each condition). Scale bar, 1 mm. GA, gravid adult; YA, young adult; L1-4, larval stage 1–4. (B and C) RNAi of v-ATPase subunits attenuated mitochondrial stress-induced lifespan extension. Survival of worms fed with control, vha-1 (20%), vha-4 (20%), vha-16 (10%), or vha-19 (20%) RNAi, with or without cco-1 (50%; B) or mrps-5 (50%; C) RNAi. (D) RNAi of ribosomal subunits attenuates mitochondrial stress-induced lifespan extension. Survival of worms fed with control, rps-8 (60%), rps-10 (60%), rpl-27 (60%), or rpl-36 (60%) RNAi, with or without cco-1 (40%) RNAi. Statistical analysis was performed by log-rank test (**P < 0.01; ***P < 0.001).