Figure S4.
RT-PCR and immunoblotting of the MASP-KO cells. (A) Transcripts of each MASP-KO cell clone were analyzed by RT-PCR. The primers were designed at the upstream and downstream regions of the deletion. (B–D) Lysates of the MASP-sKO (B), -dKO (C), and -tKO (D) cells were analyzed using rabbit anti-EpCAM mAb. Black and gray arrowheads indicate full-length and cleaved EpCAM, respectively. β-actin served as loading controls. Source data are available for this figure: SourceData FS4.