Figure S6.
Illustration of the RA coverage calculation method. To calculate RA coverage, images of the integrin (αvβ5) and paxillin (p-Pax) channels are segmented using the Robust Automatic Threshold Selection method in ImageJ. This method generates binary masks for each channel. The paxillin channel is then subtracted from the integrin channel and results in a mask containing only the RAs. RA coverage is calculated by dividing the area covered by RAs in pixels (integrated density/255) by the area covered by the cell (or cells) in an image.